Device

Part:BBa_M36414:Design

Designed by: Kelsey Pian   Group: Stanford BIOE44 - S11   (2014-10-23)


Pb (II) Sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
    Illegal PstI site found at 1112
    Illegal PstI site found at 1331
    Illegal PstI site found at 1457
    Illegal PstI site found at 1748
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 437
    Illegal PstI site found at 1112
    Illegal PstI site found at 1331
    Illegal PstI site found at 1457
    Illegal PstI site found at 1748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
    Illegal PstI site found at 1112
    Illegal PstI site found at 1331
    Illegal PstI site found at 1457
    Illegal PstI site found at 1748
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal PstI site found at 1112
    Illegal PstI site found at 1331
    Illegal PstI site found at 1457
    Illegal PstI site found at 1748
    Illegal NgoMIV site found at 1369
    Illegal NgoMIV site found at 1396
    Illegal NgoMIV site found at 1654
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that the following promoter bound to the transcription factor we chose.


Source

The promoter, RBS, and terminator regions are all standard parts of the E. coli genome. The MTF-1 and following promoter regions come from the mouse genome. The sequence was back translated from the amino acid sequence to avoid introns.

References